Bruce Wayne/Batman
Kevin Conroy
Richard `Dick' Grayson/Robin
Loren Lester
Alfred
Efrem Zimbalist Jr.
Comissioner James Gordon
Bob Hastings
Commissioner Gordon
Bob Hastings
The Joker (voice) / Ferris Boyle (voice)
Mark Hamill
Clive Revill
Summer Gleeson (voice)
Mari Devon
Detective Bullock (voice) / Detective Bullock / Rocco (voice)
Robert Costanzo
Barbara Gordon (voice) / Batgirl (voice)
Melissa Gilbert
Tara Strong
Mathew Valencia
Two-Face (voice) / Computer (voice) / Harvey Dent (voice)
Richard Moll
Rupert Thorne (voice)
John Vernon
Harley Quinn (voice)
Arleen Sorkin
Selina Kyle/Catwoman
Adrienne Barbeau
Victor Fries/Mr. Freeze
Michael Ansara
Mayor Hamilton Hill (voice)
Lloyd Bochner
Lucius Fox (voice)
Brock Peters
Poison Ivy (voice) / Mary Grayson (voice)
Diane Pershing
Matthew Hagen/Clayface
Ron Perlman
Oswald Cobblepot/The Penguin
Paul Williams
Renee Montoya (voice)
Ingrid Oliu
Roland Daggett (voice)
Edward Asner
Auctioneer (voice) / Ratso (voice) / Soldier (voice)
Neil Ross
Killer Croc (voice) / Lucas / Man (voice)
Aron Kincaid
HARDAC (voice) / Office Boy (voice) / Video Store Owner (voice)
Jeff Bennett
Ventriloquist / Scarface (voice) / G. Carl Francis (voice)
George Dzundza
Dr. Leslie Thompkins (voice)
Diana Muldaur
Ra's al Ghul (voice)
David Warner
Isis (voice) / Thug (voice) / Werewolf FX (voice)
Frank Welker
Scarecrow (voice)
Henry Polic II