Billy (Dr. Horrible)

Neil Patrick Harris

Penny

Felicia Day

Captain Hammer

Nathan Fillion

Moist

Simon Helberg

Bad Horse Chorus No. 1

Nick Towne

Bad Horse Chorus No. 2

Jed Whedon

Bad Horse Chorus No. 3

Rob Reinis

Van Driver / Driver

Otto Michael Penzato

Newswoman

Marti Noxon

Mayor

Richard Partlow

Groupie #1

Maurissa Tancharoen

Groupie #2

Stacy Shirk

Groupie #3

Steve Berg

Hawaiian Shirt Guy

Lance Lanfear

Other Moving Guy

Michael Boretz

Newsman

David Fury

Pink Pummeler

Michael G. Canaan

Professor Normal

Douglas Petrie

Fake Thomas Jefferson

Drew Goddard

Tie-Die

Kate Danson

Fury Leika

Liz Vassey

Snake Bite

Athena Demos

Director

Joss Whedon