J. Jonah Jameson (voice)

Ed Asner

Lonnie / Tombstone (voice)

Dorian Harewood

Spider-Man / Peter Parker (voice)

Christopher Daniel Barnes

Mary Jane Watson (voice)

Sara Ballantine

Alisha Silvermane (voice)

Leigh-Allyn Baker

Alistair Smythe (voice)

Maxwell Caulfield

Aunt May (voice)

Linda Gary

Harry Osborn (voice)

Gary Imhoff

Kingpin (voice)

Roscoe Lee Browne

Joe 'Robbie' Robertson (voice)

Rodney Saulsberry

Anna Watson (voice)

Majel Barrett

Matt Murdock / Daredevil (voice)

Edward Albert

Richard Fisk (voice)

Nick Jameson

Susan Choi (voice)

Amy Hill

Detective Terri Lee (voice)

Dawnn Lewis

Madame Web (voice)

Joan Lee

Landon (voice)

David Warner

Norman Osborn (voice)

Neil Ross

Randy Robertson (voice)

Alfonso Ribeiro

Martha Robertson (voice)

Iona Morris