Peter Parker / Spider-Man (voice)

Christopher Daniel Barnes

Felicia Hardy / Black Cat (voice)

Jennifer Hale

Joe 'Robbie' Robertson / Security Guard / Paramedic (voice)

Rodney Saulsberry

J Jonah Jameson (voice)

Ed Asner

Otto Octavius / Doctor Octopus / Butler (voice)

Efrem Zimbalist Jr.

Mrs. Hardy (voice)

Rue McClanahan

Brace Huntington (voice)

David Hayter

John Hardesky / The Cat (voice)

John Phillip Law

Anastasia Hardy (voice)

Nita Talbot

Kingpin (voice)

Roscoe Lee Browne

Landon (voice)

David Warner

Nick Fury (voice)

Jack Angel

Agent X (voice)

Rachel Davies

Harry Osborn (voice)

Gary Imhoff

Alistair Smythe (voice)

Maxwell Caulfield

Child Silvermane (voice)

Cannon Young

Alisha Silvermane (voice)

Leigh-Allyn Baker

Adam Toomes / Old Vulture (voice)

Eddie Albert

Scorpion (voice)

Richard Moll

Arthur Avis / Young Vulture (voice)

Alan Johnson

Sarah Baker (voice)

Sandra Bernhard

Doctor Curt Connors (voice)

Joseph Campanella

Middle-Aged Silvermane (voice)

Townsend Coleman

Silverman (voice)

Jeff Corey