Bo Duke

Jonathan Bennett

Luke Duke

Randy Wayne

Daisy Duke

April Scott

Uncle Jesse Duke

Willie Nelson

Cooter

Joel David Moore

Jefferson Davis `'Boss'` Hogg

Christopher McDonald

Rosco P. Coltrane

Harland Williams

Lulu Hogg

Sherilyn Fenn

Hughie

Todd Grinnell

Enos Strate

Adam Shulman

Ali Handy

Trishelle Cannatella

Brooke Handy

Jennifer Hill

The Balladeer (voice)

Gary Cole

Kym Stys

Shiner #2

Sy Richardson

Vanessa Motta

DMV Examiner

Rufus Dorsey

Carnival Midget

Joe Gieb

Kimberly Bosso

Patron

Doug Jones

Psychic

Challen Cates

Old Man Abernathy

Bud Mathis

Sunbathing Girl #1

Carrie Minter

Jessica Simpson

Father

Alex Boling

Tough Woman

Eve Sigall

Sunbathing Girl #2

Casey Durkin

Shiner #1

Sam Zeller

Old Shiner

Beans Morocco