Miles Morales

Shameik Moore

Peter B. Parker

Jake Johnson

Gwen Stacy (voice)

Hailee Steinfeld

Tombstone

Marvin Jones III

Brooklyn Visions Student (voice)

Mimi Davila

Uncle Aaron/The Prowler

Mahershala Ali

Brooklyn Visions Teacher (voice)

Melanie Haynes

Jefferson Davis (voice)

Brian Tyree Henry

Additional Voices (voice)

Jon Bruno

Aunt May

Lily Tomlin

Additional Voices (voice)

Darcy Rose Byrnes

Rio Morales

Luna Lauren Velez

Additional Voices (voice)

Oscar Camacho

Mary Jane

Zoë Kravitz

Additional Voices (voice)

June Christopher

Spider-Ham

John Mulaney

Additional Voices (voice)

Alycia Cooper

Peni Parker

Kimiko Glenn

Additional Voices

Terrence Hardy

Spider-Man Noir

Nicolas Cage

Additional Voices (voice)

Rif Hutton

Doc Ock

Kathryn Hahn

Wilson Fisk/Kingpin

Liev Schreiber

Additional Voices (voice)

Harrison Knight

Additional Voices (voice)

Donna Lynn Leavy

Peter Parker

Chris Pine

Additional Voices (voice)

Juan Pacheco

Miss Calleros

Natalie Morales

Additional Voices (voice)

Devika Parikh

Miss Calleros (voice)

Natalie Morales

Additional Voices (voice)

Courtney Peldon

Brooklyn Visions Security Guard (voice)

Edwin H. Bravo

Additional Voices (voice)

Chrystee Pharris

Interesting Person #1 (voice)

Oscar Isaac

Additional Voices (voice)

Juan Pope

Interesting Person #2 (voice)

Greta Lee

Additional Voices (voice)

Al Rodrigo

Stan (voice)

Stan Lee

Additional Voices (voice)

Joseph Sanfelippo

Green Goblin / Last Dude (voice)

Jorma Taccone

Additional Voices (voice)

Justin Shenkarow

Scorpion (voice)

Joaquín Cosío

Additional Voices (voice)

Warren Sroka

Tombstone (voice)

Marvin 'Krondon' Jones III

Scientist in Cafeteria (voice)

Kim Yarbrough

Additional Voices (voice)

Melissa Sturm

Additional Voices (voice)

Jason Linere-White

Vanessa Fisk (voice)

Lake Bell

Additional Voices (voice)

Ruth Zalduondo

Brooklyn Friend (voice)

Jessica Mikayla Adams

Brooklyn Friend (voice)

Gredel Berrios Calladine

Brooklyn Friend (voice)

Sarah D. Cole

Brooklyn Friend (voice)

Kelby Joseph

Brooklyn Visions Teacher (voice)

Claudia Choi

Brooklyn Visions Teacher (voice)

Joseph Izzo

Brooklyn Visions Teacher (voice)

Nick Jaine

Brooklyn Visions Teacher (voice)

Muneeb Rehman

Brooklyn Visions Teacher (voice)

Carlos Zaragoza

Brooklyn Bystander (voice)

Post Malone

Additional Voices (voice)

David Applebee

Additional Voices (voice)

Juan Carlos Arvelo

Additional Voices (voice)

Adam Brown

Scorpion

Joaquín Cosio*

Additional Voices (voice)

Michelle Jubilee Gonzalez

Additional Voices (voice)

Terrence Hardy Jr.*

Additional Voices (voice)

Bridget Hoffman

Additional Voices (voice)

Miguel Jiron

Additional Voices (voice)

Lex Lang

Additional Voices (voice)

Andrew Leviton

Additional Voices (voice)

Caitlin McKenna-Wilkinson

Additional Voices (voice)

Scott Menville

Additional Voices (voice)

Christopher Miller

Additional Voices (voice)

Arthur Ortiz

Additional Voices (voice)

Shakira Ja'nai Paye

Additional Voices (voice)

Jacqueline Pinol

Additional Voices (voice)

Dennis Singletary

Additional Voices (voice)

Holly Walker

Director

Rodney Rothman

Director

Peter Ramsey

Director

Bob Persichetti