Miles Morales

Ronnie Flex

Miles Morales

Shameik Moore

Peter B. Parker

Jake Johnson

Peter B. Parker

Thijs Römer

Gwen Stacy (voice)

Hailee Steinfeld

Tombstone

Marvin Jones III

Spider-Gwen

Holly Mae Brood

Rio Morales

Lauren Velez

Brooklyn Visions Student (voice)

Mimi Davila

Uncle Aaron/The Prowler

Mahershala Ali

Jefferson David

Edwin Jonker

Brooklyn Visions Teacher (voice)

Melanie Haynes

Jefferson Davis

Brian Tyree Henry

RIPeter

Levi van Kempen

Additional Voices (voice)

Jon Bruno

Oom Aaron

Fernando Halman

Aunt May

Lily Tomlin

Doc Ock

Kim van Kooten

Additional Voices (voice)

Darcy Rose Byrnes

Additional Voices (voice)

Oscar Camacho

Spider-Man Noir en Buddy Vedder: Spider-Ham

Frank Lammers

Mary Jane

Zoë Kravitz

Additional Voices (voice)

June Christopher

Spider-Ham

John Mulaney

Wilson Fisk

Derek de Lint

Additional Voices (voice)

Alycia Cooper

Peni Parker

Kimiko Glenn

Additional Voices

Terrence Hardy

Spider-Man Noir

Nicolas Cage

Additional Voices (voice)

Rif Hutton

Doc Ock

Kathryn Hahn

Wilson Fisk/Kingpin

Liev Schreiber

Additional Voices (voice)

Harrison Knight

Additional Voices (voice)

Donna Lynn Leavy

Peter Parker

Chris Pine

Additional Voices (voice)

Juan Pacheco

Miss Calleros

Natalie Morales

Additional Voices (voice)

Devika Parikh

Additional Voices (voice)

Courtney Peldon

Brooklyn Visions Security Guard (voice)

Edwin H. Bravo

Additional Voices (voice)

Chrystee Pharris

Interesting Person #1 (voice)

Oscar Isaac

Additional Voices (voice)

Juan Pope

Interesting Person #2 (voice)

Greta Lee

Additional Voices (voice)

Al Rodrigo

Stan (voice)

Stan Lee

Additional Voices (voice)

Joseph Sanfelippo

Green Goblin / Last Dude (voice)

Jorma Taccone

Scorpion (voice)

Joaquín Cosío

Additional Voices (voice)

Justin Shenkarow

Additional Voices (voice)

Warren Sroka

Tombstone (voice)

Marvin 'Krondon' Jones III

Scientist in Cafeteria (voice)

Kim Yarbrough

Additional Voices (voice)

Melissa Sturm

Additional Voices (voice)

Jason Linere-White

Vanessa Fisk (voice)

Lake Bell

Additional Voices (voice)

Ruth Zalduondo

Brooklyn Friend (voice)

Jessica Mikayla Adams

Brooklyn Friend (voice)

Gredel Berrios Calladine

Brooklyn Friend (voice)

Sarah D. Cole

Brooklyn Friend (voice)

Kelby Joseph

Brooklyn Visions Teacher (voice)

Claudia Choi

Brooklyn Visions Teacher (voice)

Joseph Izzo

Brooklyn Visions Teacher (voice)

Nick Jaine

Brooklyn Visions Teacher (voice)

Muneeb Rehman

Brooklyn Visions Teacher (voice)

Carlos Zaragoza

Brooklyn Bystander (voice)

Post Malone

Additional Voices (voice)

David Applebee

Additional Voices (voice)

Juan Carlos Arvelo

Additional Voices (voice)

Adam Brown

Additional Voices (voice)

Michelle Jubilee Gonzalez

Additional Voices (voice)

Terrence Hardy Jr.*

Additional Voices (voice)

Bridget Hoffman

Additional Voices (voice)

Miguel Jiron

Additional Voices (voice)

Lex Lang

Additional Voices (voice)

Andrew Leviton

Additional Voices (voice)

Caitlin McKenna-Wilkinson

Additional Voices (voice)

Scott Menville

Additional Voices (voice)

Christopher Miller

Additional Voices (voice)

Arthur Ortiz

Additional Voices (voice)

Shakira Ja'nai Paye

Additional Voices (voice)

Jacqueline Pinol

Additional Voices (voice)

Dennis Singletary

Additional Voices (voice)

Holly Walker

Brooklyn Visions Security Guard

Edwin Bravo

Tombstone

Marvin 'Krondon' Jones, lll