Miles Morales (voice)

Shameik Moore

Gwen Stacy (voice)

Hailee Steinfeld

Jefferson Morales (voice)

Brian Tyree Henry

Rio Morales (voice)

Luna Lauren Vélez

Miguel O'Hara (voice)

Oscar Isaac

Peter B. Parker (voice)

Jake Johnson

Spot (voice)

Jason Schwartzman

Jessica Drew (voice)

Issa Rae

Hobie Brown (voice)

Daniel Kaluuya

Spider-Man Noir (voice)

Nicolas Cage

Spider-Ham (voice)

John Mulaney

Pavitr Prabhakar (voice)

Karan Soni

Peni Parker (voice)

Kimiko Glenn

Margo Kess (voice)

Amandla Stenberg

Uncle Aaron (voice)

Mahershala Ali

Miles G. Morales (voice)

Jharrel Jerome

LYLA (voice)

Greta Lee

Ben Reilly (voice)

Andy Samberg

George Stacy (voice)

Shea Whigham

Tombstone (voice)

Marvin Jones III

Wilson Fisk (voice)

Liev Schreiber