The Nerd / Batman / Robin / Aquaman / The Penguin / Martian Manhunter / Toyman / Scarecrow / Gorilla Grodd / Little Cheese / Swamp Thing / Abin Sur / Ganthet / Green Arrow / Hawkman / Jimmy Olsen / Various (voice)

Seth Green

Green Lantern / Mr. Freeze (voice)

Nathan Fillion

Superman / Mirror Master (voice)

Breckin Meyer

Wonder Woman / Giganta / Woman (voice)

Alex Borstein

Lois Lane (voice)

Megan Fox

Two-Face / Black Manta (voice)

Neil Patrick Harris

The Flash / Chillblaine / Brainiac / Wildcat (voice)

Matthew Senreich

Lex Luthor / Firestorm / Mr. Banjo (voice)

Alfred Molina

The Riddler (voice)

Paul Reubens

Mister Banjo / Glenn (voice)

Aaron Paul

Narrator / Captain Cold (voice)

Kevin Shinick

Cyborg/ Kilowog / Appa Ali Apsa / Solomon Grundy (voice)

Abraham Benrubi

Harley Quinn / Selena Gomez (voice)

Tara Strong

Sinestro / Swamp Thing (voice)

Zeb Wells

Icicle/B'dg (voice)

Tom Root