Basil of Baker Street / Bartholomew (voice)

Barrie Ingham

Professor Ratigan (voice)

Vincent Price

Dr. David Q. Dawson / Thug Guard (voice)

Val Bettin

Olivia Flaversham

Susan Pollatschek

The Mouse Queen (voice)

Eve Brenner

Olivia Flaversham

Susanne Pollatschek

Hiram Flaversham (voice)

Alan Young

Fidget

Candy Candido

Mrs. Judson

Diana Chesney

Sherlock Holmes (voice)

Basil Rathbone

Citizen

Walter Edmiston

Toby the Dog / Felicia the Cat (voice) (uncredited)

Frank Welker

Dr. Watson (archive sound)

Laurie Main

Miss Kitty Mouse (voice) (uncredited)

Melissa Manchester

Bar Maid (voice)

Ellen Fitzhugh

Lady Mouse

Shani Wallis

Citizen / Thug Guard (voice)

Walker Edmiston

(voice) (uncredited)

Charles Fleischer

Thug Guard (voice)

Wayne Allwine

Thug Guard (voice)

Tony Anselmo