Peter Parker/Spider-Man

Drake Bell

Nick Fury

Chi McBride

Aunt May Parker (voice)

Misty Lee

J. Jonah Jameson

J.K. Simmons

Stan the Janitor

Stan Lee

Agent Coulson

Clark Gregg

Luke Cage / Power Man (voice)

Ogie Banks

Norman Osborn

Steven Weber

Danny Rand/Iron Fist

Greg Cipes

Sam Alexander / Nova (voice)

Logan Miller

Dr. Otto Octavius / Bentley Wittman (voice) / Doctor Octopus

Tom Kenny

Harry Osborn (voice) / Flash Thompson / Agent Venom

Matt Lanter

Ava Ayala/White Tiger

Caitlyn Taylor Love

Prowler (voice)

Tyler James Williams

Mary-Jane Watson

Tara Strong

Flash Thompson (voice)

Dante Basco

Miles Morales

Donald Glover

Nightmare

Mark Hamill

Spider-Noir

Milo Ventimiglia

Dagger (voice)

Ashley Eckstein

Spider-Girl

Olivia Holt

Norma Osbourne

Wendie Malick

Spider-Man from 2099

Freddy Rodriguez

Taskmaster (voice)

Clancy Brown

Bone Spider / Goliath Spider (voice)

Imari Williams

Wolverine (voice) / Wolverine / Ka Zar (voice)

Steve Blum

Ant-Man (voice) / Scott Lang

Grant George