Peter Parker / Spider-Man (voice)
Drake Bell
Nick Fury
Chi McBride
Aunt May Parker (voice)
Misty Lee
J. Jonah Jameson (voice)
J.K. Simmons
Stan the Janitor
Stan Lee
Agent Coulson
Clark Gregg
Luke Cage / Power Man (voice)
Ogie Banks
Norman Osborn
Steven Weber
Danny Rand/Iron Fist
Greg Cipes
Prowler (voice)
Tyler James Williams
Ava Ayala / White Tiger (voice)
Caitlyn Taylor Love
Sam Alexander/Nova
Logan Miller
Dr. Otto Octavius / Bentley Wittman (voice)
Tom Kenny
Flash Thompson (voice)
Dante Basco
Harry Osborn/Flash Thompson
Matt Lanter
Mary-Jane Watson
Tara Strong
Miles Morales
Donald Glover
Arnim Zola (voice) / Nightmare / Shao Lao (voice)
Mark Hamill
Spider-Noir
Milo Ventimiglia
Dagger (voice)
Ashley Eckstein
Spider-Girl
Olivia Holt
Norma Osbourne
Wendie Malick
Bone Spider / Goliath Spider (voice)
Imari Williams
Spider-Man from 2099
Freddy Rodriguez
Jack Russell / Werewolf by Night (voice)
Ross Lynch
Captain Ultra / Piledriver (voice)
Cam Clarke
Korvac / Chitauri #3 (voice)
James Marsters