Peter Parker / Spider-Man (voice)

Drake Bell

Nick Fury

Chi McBride

Aunt May Parker (voice)

Misty Lee

J. Jonah Jameson (voice)

J.K. Simmons

Stan the Janitor

Stan Lee

Agent Coulson

Clark Gregg

Luke Cage / Power Man (voice)

Ogie Banks

Norman Osborn

Steven Weber

Danny Rand/Iron Fist

Greg Cipes

Prowler (voice)

Tyler James Williams

Ava Ayala / White Tiger (voice)

Caitlyn Taylor Love

Sam Alexander/Nova

Logan Miller

Dr. Otto Octavius / Bentley Wittman (voice)

Tom Kenny

Flash Thompson (voice)

Dante Basco

Harry Osborn/Flash Thompson

Matt Lanter

Mary-Jane Watson

Tara Strong

Miles Morales

Donald Glover

Arnim Zola (voice) / Nightmare / Shao Lao (voice)

Mark Hamill

Spider-Noir

Milo Ventimiglia

Dagger (voice)

Ashley Eckstein

Spider-Girl

Olivia Holt

Norma Osbourne

Wendie Malick

Bone Spider / Goliath Spider (voice)

Imari Williams

Spider-Man from 2099

Freddy Rodriguez

Jack Russell / Werewolf by Night (voice)

Ross Lynch

Captain Ultra / Piledriver (voice)

Cam Clarke

Korvac / Chitauri #3 (voice)

James Marsters