Peter Parker/Spiderman

Josh Keaton

Gwen Stacy

Lacey Chabert

Flash Thompson (voice) / Flash Thompson / Car Thief #1 (voice)

Joshua LeBar

Betty Brant/Sally Avril

Grey Griffin

Liz Allan (voice) / Liz Allan / Trina (voice)

Alanna Ubach

Mary Jane Watson (voice) / Mary Jane Watson / Rosie Thompson (voice)

Vanessa Marshall

Harry Osborn (voice) / Harry Osborn / Frederick Foswell (voice)

James Arnold Taylor

Shocker/St. John Devereaux

Jeff Bennett

Curt Connors (voice) / Curt Connors / Lizard (voice)

Dee Bradley Baker

Eddie Brock (voice) / Venom (voice) / Eddie Brock

Ben Diskin

Irene Bedard

Irene Bedard

May Parker (voice)

Deborah Strang

Electro (voice) / Max Dillon / Thug #1 (voice)

Crispin Freeman

Tombstone (voice) / Principal Davis (voice) / Tombstone

Kevin Michael Richardson

Rand Robertson (voice) / Rand Robertson / Robbie Robertson (voice)

Phil LaMarr

Quentin Beck

Xander Berkeley

George Stacy (voice) / Rhino / Alex O'Hirn (voice)

Clancy Brown

Otto Octavius (voice) / Doctor Octopus (voice) / Master Planner (voice)

Peter MacNicol

Hammerhead (voice) / Sandman / Flint Marko

John DiMaggio

Dilbert Trillby/Chameleon

Steve Blum

Norman Osborn (voice)

Alan Rachins

J. Jonah Jameson (voice) / J. Jonah Jameson / John Jameson (voice)

Daran Norris

Glory Grant (voice) / Glory Grant / Cobweb

Cree Summer

Vulture (voice) / Adrian Toomes

Robert Englund

Black Cat (voice) / Felicia Hardy

Tricia Helfer

Director

Victor Cook

Director

Kevin Altieri