Batman/Bruce Wayne

Anthony Ruivivar

Alfred Pennyworth (voice)

JB Blanc

Lt. James Gordon

Kurtwood Smith

Katana

Sumalee Montano

Police Commissioner/Officer Rodriguez

Carlos Ferro

Street Thug

Abraham Benrubi

Killer Croc / Waylon Jones (voice) / Ernie Croskey (voice)

Wade Williams

Mutant Ninja 1/Mutant Ninja 2

Dee Bradley Baker

Junkyard Dog

Carlos Alazraqui

Phosphorous Rex/Milo Match

Greg Ellis

Metamorpho / Rex Mason (voice)

Adam Baldwin

Joe Braxton/Police Officer

Ian James Corlett

Ava Kirk/Gloria Pearce

Tisha T. Banker

Humpty Dumpty/Humphrey Dumpler

Matt Jones

Anarky / Lonnie Machin (voice) / Lonnie Machin

Wallace Langham

Dr. Jason Burr (voice)

Matthew Lillard

Lady Shiva

Finola Hughes

Mayor Marion Grange

CCH Pounder

Barbara Gordon (voice) / Oracle / Barbara Gordon

Tara Strong

Simon Stagg (voice) / Simon Stagg / Store Owner

Jeff Bennett

Professor Pyg (voice) / Professor Pyg / Liam Taylor (voice)

Brian George

Officer O'Brien (voice) / Officer O'Brien / Thug (voice)

Michael Patrick McGill

Deathstroke/Slade Wilson/Man-Bat/Kirk Langstrom

Robin Atkin Downes

William Benjamin/Officer Dunbrowski/Subway Conductor

James Arnold Taylor

Silver Monkey (voice) / Silver Monkey / Mr. Reese (voice)

James Remar

Magpie

Grey Griffin

Daedalus Boch

Arif S. Kinchen

Guard

Gary Sturgis

Manhunter/Paul Kirk

Xander Berkeley

Sapphire Stagg

Emmanuelle Chriqui

Bethanie Ravencroft (voice)

Cree Summer

Creed Courtman/Detective Thomas

Yuri Lowenthal

Tobias Whale

Michael Leon Wooley

Ice Pick Joe

Matthew Mercer

Mister Toad (voice)

Udo Kier

Harvey Dent (voice) / Harvey Dent / Weapons Seller (voice)

Christopher McDonald

Dr. Anatol Mykros

Bruce Thomas

David Hull/Mercenery

James Patrick Stuart

Ra's Al Ghul (voice)

Lance Reddick

Michael Holt

Gary Anthony Williams

Young Bruce Wayne

Jason Marsden

Jocelyn Kilroy

Beth Tapper

Edogawa Yamashiro

Paul Nakauchi

Diner Owner

Laraine Newman