Alice

Milla Jovovich

Carlos Olivera

Oded Fehr

Claire

Ali Larter

Dr. Isaacs

Iain Glen

Nurse Betty

Ashanti

Mikey

Christopher Egan

K-Mart

Spencer Locke

Slater

Matthew Marsden

L.J.

Mike Epps

Chase

Linden Ashby

Albert Wesker

Jason O'Mara

Otto

Joe Hursley

Umbrella Tech

John Eric Bentley

Lab Tech

James Tumminia

White Queen

Madeline Carroll

Scientist

Kirk B.R. Woller

Ice Hockey / Corridor Guard

Rick Cramer

Ma

Valorie Hubbard

Rancid / Tyrant

Brian Steele

British Envoy

Peter O'Meara

French Envoy

William Abadie

Runty

Ramón Franco

Piggy

Shane Woodson

Pock Mark

Geoff Meed

Eddie

Rusty Joiner

Small Boy

Connor McCoy

Mirror Woman

Carolina Jimenez

Tyrant Vocal (as Gary Hecker)

Gary A. Hecker

Additional Voices (voice) (uncredited)

Richard Epcar

Umbrella Captain (uncredited)

Gary Hudson

Sienna Guillory

Zombie Steve (uncredited)

Carsten Nørgaard

Chris Durand

Foreign Envoy (uncredited)

César Ámigo Aguilar

Jeremy Bolt

Robert Kulzer