Mary Stuart

Katharine Hepburn

Bothwell

Fredric March

Elizabeth Tudor

Florence Eldridge

Darnley

Douglas Walton

Rizzio

John Carradine

Morton

Robert Barrat

Earl of Leicester

Gavin Muir

Moray

Ian Keith

John Knox

Moroni Olsen

Lord Ruthven

William Stack

Lord Randolph

Ralph Forbes

Throckmorton

Alan Mowbray

Mary Beaton

Frieda Inescort

Huntley

Donald Crisp

Lindsay

David Torrence

Mary Livingstone

Molly Lamont

Burghley

Lionel Pape

Mary Fleming

Anita Colby

Mary Seton

Jean Fenwick

Donal

Alec Craig

Nurse

Mary Gordon

Messenger

Monte Blue

Maitland

Leonard Mudie

Sir Francis Knollys

Robert Warwick

Arian

Brandon Hurst

Man (uncredited)

Halliwell Hobbes

Lexington

Wilfred Lucas

Judge

Nigel De Brulier

Kirkcaldy

D'Arcy Corrigan

Douglas

Frank Baker

Faudoncide

Cyril McLaglen

English Fisherman

Lionel Belmore

Fisherman's Wife

Doris Lloyd

His Son

Bobby Watson

Judge

Ivan Simpson

Judge

Murray Kinnell

Judge

Lawrence Grant

Judge

Barlowe Borland

Sir Francis Walsingham

Walter Byron

Sergeant-at-Arms

Wyndham Standing

Earl of Kent

Earle Foxe

Du Croche

Paul McAllister

Chatelard

Gaston Glass

Nobleman

Neil Fitzgerald

Prince Jame

Jean Kirchner

Prince Jame

Judith Kirchner

Jailer

Robert E. Homans

Morton

Robert H. Barrat

Earl of Kent

Earl Foxe