Scooby-Doo / Fred Jones / Bikers (voice)
Frank Welker
Shaggy Rogers / Shaky Joe (voice)
Matthew Lillard
Velma Dinkley
Mindy Cohn
Daphne Blake
Grey DeLisle
Faith (voice)
Cathy Cavadini
Emily
Vyvan Pham
Mr. Hubley (voice)
Fred Willard
Winsor
Matthew Gray Gubler
Mr. Babbit / Blair / Grad Student #1 / Bikers (voice)
Michael Gough
Professor Svankmajer (voice)
Finola Hughes
Ms. Deitch (voice)
Gwendoline Yeo
Professor Svankmajer
Finola Hughes*
Doctor
Maulik Pancholy
Cleo
Colleen O'Shaughnessey
Tex / Cop #2 / Grad Student #3 / Bikers (voice)
Kevin Michael Richardson
Friz / GPS / Bikers (voice)
John DiMaggio
Ethan Spaulding
Robert J. Kral
Policeman / Cop #1 / Grad Student #2 / Bikers (voice)
Dave Wittenberg
Sam Register*
Doug Langdale
Sumalee Montano
Nick Palatas
Thea White
Frank Welker