Scooby Doo/Fred Jones/Bikers

Frank Welker

Shaggy Rogers / Shaky Joe (voice)

Matthew Lillard

Velma Dinkley

Mindy Cohn

Daphne Blake

Grey DeLisle

Faith (voice)

Cathy Cavadini

Emily

Vyvan Pham

Mr. Hubley (voice)

Fred Willard

Professor Svankmajer (voice)

Finola Hughes

Friz / GPS / Msicellaneous Bikers (voice)

John DiMaggio

Winsor

Matthew Gray Gubler

Mr. Babbit / Blair / Grad Student #1 / Bikers (voice)

Michael Gough

Ms. Deitch (voice)

Gwendoline Yeo

Doctor (voice)

Maulik Pancholy

Cleo

Colleen O'Shaughnessey

Tex / Cop #2 / Grad Student #3 / Bikers (voice)

Kevin Michael Richardson

Policeman / Cop #1 / Grad Student #2 / Bikers (voice)

Dave Wittenberg

Ethan Spaulding

Robert J. Kral

Sam Register*

Doug Langdale

Sumalee Montano

Nick Palatas

Thea White