Bruce Wayne/Batman

Kevin Conroy

Tim Drake/Robin

Mathew Valencia

Barbara Gordon/Batgirl

Tara Strong

Summer Gleeson (voice)

Mari Devon

Dick Grayson/Nightwing

Loren Lester

Detective Bullock (voice) / Detective Bullock / Rocco (voice)

Robert Costanzo

The Joker (voice) / Ferris Boyle (voice)

Mark Hamill

Commissioner James Gordon

Bob Hastings

Two-Face (voice) / Computer (voice) / Harvey Dent (voice)

Richard Moll

Rupert Thorne (voice)

John Vernon

Harley Quinn (voice)

Arleen Sorkin

Mayor Hill (voice)

Lloyd Bochner

Lucius Fox (voice)

Brock Peters

Poison Ivy (voice) / Mary Grayson (voice)

Diane Pershing

Catwoman (voice)

Adrienne Barbeau

Penguin (voice)

Paul Williams

Renee Montoya (voice)

Ingrid Oliu

Dishwasher / Doctor / Criminal (voice)

Neil Ross

Killer Croc (voice) / Lucas / Man (voice)

Aron Kincaid

HARDAC (voice) / Office Boy (voice) / Batcave Computer (voice)

Jeff Bennett

Barbara Gordon (voice) / Batgirl (voice)

Melissa Gilbert

Ventriloquist / Scarface (voice) / G. Carl Francis (voice)

George Dzundza

Dr. Leslie Thompkins (voice)

Diana Muldaur

Ra's al Ghul (voice)

David Warner

Roland Daggett (voice)

Ed Asner

Isis (voice) / Werewolf FX (voice) / Thug (voice)

Frank Welker

Scarecrow (voice)

Henry Polic II