Peter Parker / Spider-Man (voice)

Christopher Daniel Barnes

Mary Jane Watson (voice)

Sara Ballantine

Felicia Hardy (voice)

Jennifer Hale

Aunt May Parker (voice)

Linda Gary

Harry Osborn (voice)

Gary Imhoff

J. Jonah Jameson (voice)

Edward Asner

Joe "Robbie" Robertson (voice)

Rodney Saulsberry

Kingpin (voice)

Roscoe Lee Browne

Michael Morbius (voice) / Richard Fisk (voice) / Morbius (voice)

Nick Jameson

Anna Watson (voice)

Majel Barrett

Alistair Smythe (voice) / Ultimate Slayer Smythe (voice)

Maxwell Caulfield

Norman Osborn (voice) / Norman Osborn / Green Goblin (voice)

Neil Ross

Debra Whitman (voice)

Liz Georges

Flash Thompson (voice)

Patrick Labyorteaux

Detective Terri Lee (voice)

Dawnn Lewis

Landon (voice)

David Warner

Doctor Octopus (voice) / Otto Octavius

Efrem Zimbalist Jr.

Madame Web (voice)

Joan Lee

Newscaster (voice) / News Reporter (voice) / News Announcer (voice)

John Beard

Shocker (voice) / Man-Spider (voice)

Jim Cummings

Beyonder (voice) / Red Skull (voice)

Earl Boen

Eddie Brock / Venom (voice) / Eddie Brock (voice)

Hank Azaria

Hobgoblin (voice) / Jason Phillips / Jason Phillips (voice)

Mark Hamill

Dr. Curt Connors / Lizard (voice)

Joseph Campanella

Rhino (voice)

Don Stark

Kraven (voice) / Quentin Beck / Mysterio (voice)

Gregg Berger

Liz Allen (voice)

Marla Rubinoff

Captain America (voice) / Brace Huntington (voice)

David Hayter

Arthur Avis / Young Vulture (voice) / Vulture (voice)

Alan Johnson

Dr. Mariah Crawford (voice) / Dr. Mariah Crawford / Calypso (voice)

Susan Beaubian

Scorpion (voice)

Richard Moll

Tony Stark / Iron Man (voice)

Robert Hays

Scorpion (voice) / Mac Gargan

Martin Landau

Storm (voice) / Martha Robertson (voice)

Iona Morris

Silvermane (voice)

Jeff Corey

Nick Fury (voice)

Jack Angel

Director

Bob Richardson