Bruce Wayne/Batman

Rino Romano

D.A.V.E.

Jeff Bennett

Dick Grayson/Robin

Evan Sabara

The Joker (voice)

Kevin Michael Richardson

Batgirl / Barbara Gordon (voice)

Danielle Judovits

Gearhead

Will Friedle

Alfred Pennyworth (voice)

Alastair Duncan

Detective Ethan Bennett (voice) / Ethan Bennett / Clayface (voice)

Steve Harris

Detective Ellen Yin

Ming-Na

Chief Rojas

Edward James Olmos

The Penguin (voice)

Tom Kenny

Catwoman (voice)

Gina Gershon

Commissioner Gordon (voice) / Gordon (voice)

Mitch Pileggi

Mr. Freeze (voice) / Lex Luthor (voice)

Clancy Brown

Chief Rojas (voice)

Jesse Corti

Mayor Grange (voice)

Adam West

Hugo Strange (voice) / Toyman

Richard Green

Firefly (voice) / Boy #1 / Boy #2 (voice)

Jason Marsden

Martian Manhunter (voice)

Dorian Harewood

Killer Croc (voice) / Rumor

Ron Perlman

Superman (voice)

George Newbern

Clayface / Metallo (voice) / Riddleman #1 (voice)

Lex Lang

The Riddler (voice)

Robert Englund