The Batman / Bruce Wayne (voice)

Rino Romano

Cluemaster

Glenn Shadix

Dick Grayson/Robin

Evan Sabara

Batgirl / Barbara Gordon (voice)

Danielle Judovits

Alfred Pennyworth

Alastair Duncan

The Joker (voice)

Kevin Michael Richardson

Detective Ellen Yin (voice)

Ming-Na Wen

Detective Ethan Bennett (voice) / Ethan Bennett / Clayface (voice)

Steve Harris

Chief Rojas

Edward James Olmos

Commissioner Gordon (voice) / Gordon (voice)

Mitch Pileggi

The Penguin (voice)

Tom Kenny

Chief Rojas (voice)

Jesse Corti

Mayor Grange (voice)

Adam West

Catwoman (voice)

Gina Gershon

Mr. Freeze (voice) / Lex Luthor (voice)

Clancy Brown

Hugo Strange (voice) / Toyman

Richard Green

Dr. Langstrom

Peter MacNicol

Firefly (voice) / Boy #1 / Boy #2 (voice)

Jason Marsden

Martian Manhunter (voice)

Dorian Harewood

Killer Croc (voice) / Rumor

Ron Perlman

Superman (voice)

George Newbern

Clayface / Metallo (voice) / Riddleman #1 (voice)

Lex Lang

The Riddler (voice)

Robert Englund