Bruce Wayne/Batman
Rino Romano
Dick Grayson/Robin
Evan Sabara
Alfred Pennyworth (voice)
Alastair Duncan
Batgirl / Barbara Gordon (voice)
Danielle Judovits
Detective Ellen Yin (voice)
Ming-Na Wen
The Joker (voice)
Kevin Michael Richardson
Detective Ethan Bennett (voice) / Ethan Bennett / Clayface (voice)
Steve Harris
Chief Rojas
Edward James Olmos
Commissioner Gordon (voice) / Gordon (voice)
Mitch Pileggi
The Penguin (voice)
Tom Kenny
Chief Rojas (voice)
Jesse Corti
Mayor Grange (voice)
Adam West
Catwoman (voice)
Gina Gershon
Mr. Freeze (voice) / Lex Luthor (voice)
Clancy Brown
Hugo Strange (voice) / Toyman
Richard Green
Dr. Langstrom
Peter MacNicol
Firefly (voice) / Boy #1 / Boy #2 (voice)
Jason Marsden
Martian Manhunter (voice)
Dorian Harewood
Killer Croc (voice) / Rumor
Ron Perlman
Superman (voice)
George Newbern
Clayface / Metallo (voice) / Riddleman #1 (voice)
Lex Lang
The Riddler (voice)
Robert Englund