Nick
Nick Kroll
Andrew Glouberman (voice)
John Mulaney
Caleb (voice) / Caleb / Lump Humpman (voice)
Joe Wengert
Diane
Maya Rudolph
Jessi Glaser (voice)
Jessi Klein
Marty Glouberman (voice)
Richard Kind
Jay
Jason Mantzoukas
Ghost of Duke Ellington (voice)
Jordan Peele
Lars (voice) / Lars / Boy (voice)
Neil Casey
Elliot
Fred Armisen
Barbara Glouberman (voice) / Barbara Glouberman / Bubbe (voice)
Paula Pell
Missy Foreman-Greenwald (voice)
Jenny Slate
Jessica Chaffin
Greg Glaser (voice) / (voice) / Ninth Grader (voice)
Seth Morris
Matthew MacDell (voice)
Andrew Rannells
DeVon (voice) / DeVon / Gina's Brother #2 (voice)
Jak Knight
Devin (voice)
June Diane Raphael
Judd Birch (voice) / Judd Birch / Pumbaa (voice)
Jon Daly
Missy Foreman-Greenwald (voice) / Missy Foreman-Greenwald / Mosaic Missy (voice)
Ayo Edibiri
Leah Birch (voice) / Leah Birch / Lorraine the Hormone Monstress (voice)
Kat Dennings
Gina Alvarez (voice) / Gina Alvarez / Gina Parrot (voice)
Gina Rodriguez
Monica Foreman-Greenwald (voice) / Cellsea (voice) / Diaphragm (voice)
Chelsea Peretti
Jenna Bilzerian (voice)
Heather Lawless
(voice) / Vendor (voice) / Backup Singer (voice)
Mark Rivers
(voice) / Brad (voice) / Wiggles (voice)
Gil Ozeri
Walter the Lovebug (voice) / Walter (voice)
Brandon Kyle Goodman
Val Bilzerian (voice)
Mark Duplass
Shame Wizard (voice)
David Thewlis
Nathan Fillion (voice)
Nathan Fillion
Aiden (voice)
Zachary Quinto
Vicky (voice) / Milk (voice) / Olivia
Emily Altman
Mr. Lizer (voice)
Rob Huebel
Kurt Bilzerian (voice)
Paul Scheer
Tyler the Hormone Monster (voice) / Security Guard / Bus Driver's Son (voice)
John Gemberling
Cantor Dina Reznick (voice)
Michaela Watkins
(voice) / Girl (voice) / Maddie the American Girl Doll
Fran Gillespie
Ali (voice)
Ali Wong
Tito the Anxiety Mosquito (voice)
Maria Bamford
Depression Kitty (voice)
Jean Smart
Pubic Hair #1 (voice)
Jack McBrayer