Nick

Nick Kroll

Andrew

John Mulaney

Diane Birch / Constance the Hormone Monstress (voice)

Maya Rudolph

Caleb (voice) / Caleb / Lump Humpman (voice)

Joe Wengert

Jessi Glaser (voice)

Jessi Klein

Marty Glouberman (voice)

Richard Kind

Jay

Jason Mantzoukas

Ghost of Duke Ellington

Jordan Peele

Lars (voice) / Lars / Boy (voice)

Neil Casey

Elliot

Fred Armisen

Barbara Glouberman (voice) / Barbara Glouberman / Bubbe (voice)

Paula Pell

Missy

Jenny Slate

Jessica Chaffin

Greg Glaser (voice) / (voice) / Ninth Grader (voice)

Seth Morris

Matthew MacDell (voice)

Andrew Rannells

DeVon (voice) / DeVon / Gina's Brother #2 (voice)

Jak Knight

Devin (voice)

June Diane Raphael

Judd Birch (voice) / Judd Birch / Pumbaa (voice)

Jon Daly

Missy Foreman-Greenwald (voice) / Missy Foreman-Greenwald / Mosaic Missy (voice)

Ayo Edibiri

Leah Birch (voice) / Leah Birch / Lorraine the Hormone Monstress (voice)

Kat Dennings

Gina Alvarez (voice) / Gina Alvarez / Gina Parrot (voice)

Gina Rodriguez

Monica Foreman-Greenwald (voice) / Cellsea (voice) / Diaphragm (voice)

Chelsea Peretti

Jenna Bilzerian (voice)

Heather Lawless

(voice) / Vendor (voice) / Backup Singer (voice)

Mark Rivers

(voice) / Brad (voice) / Wiggles (voice)

Gil Ozeri

Walter the Lovebug (voice) / Walter (voice)

Brandon Kyle Goodman

Val Bilzerian (voice)

Mark Duplass

Shame Wizard (voice)

David Thewlis

Nathan Fillion (voice)

Nathan Fillion

Aiden (voice)

Zachary Quinto

Vicky (voice) / Milk (voice) / Olivia

Emily Altman

Mr. Lizer (voice)

Rob Huebel

Kurt Bilzerian (voice)

Paul Scheer

Tyler the Hormone Monster (voice) / Security Guard / Bus Driver's Son (voice)

John Gemberling

Cantor Dina Reznick (voice)

Michaela Watkins

(voice) / Girl (voice) / Maddie the American Girl Doll

Fran Gillespie

Ali (voice)

Ali Wong

Tito the Anxiety Mosquito (voice)

Maria Bamford

Depression Kitty (voice)

Jean Smart

Pubic Hair #1 (voice)

Jack McBrayer