Spider-Man

Robbie Daymond

Harry Osborn

Max Mittelman

Doc Ock

Scott Menville

Miles Morales (voice) / Security Robot (voice)

Nadji Jeter

Anya Corazon (voice) / Chaperone (voice) / Female Clerk (voice)

Melanie Minichino

Max Modell (voice) / Trucker / Mr. Slott (voice)

Fred Tatasciore

Flash Thompson / Student #2 (voice) / Flash Thompson (voice)

Ben Diskin

Ben Pronsky

Aunt May (voice) / Woman Bystander (voice) / Bomb Voice (voice)

Nancy Linari

Gwen Stacy (voice) / Osborn Recruiter #2 (voice)

Laura Bailey

Uncle Ben (voice)

Patton Oswalt

Raymond Warren (voice) / Security Guard / Customer (voice)

John DiMaggio

Norman Osborn (voice) / Prison Guard (voice) / Hologram (voice)

Josh Keaton

Vulture

Alastair Duncan

Bob Joles

Joe

Joe Quesada

Alistair Smythe (voice) / Audience Member (voice) / Alistair Smythe

Jason Spisak

Hammerhead (voice)

Jim Cummings

Yuri Lowenthal

Herman Schultz / Shocker (voice)

Cameron Boyce

Sand Girl (voice) / Keemia (voice)

Sofia Carson

Aleksei Sytsevich / Comic Book Guy (voice) / Rhino

Matthew Mercer

Prowler (voice)

Nathaniel J. Potvin

Police Officer #3 (voice)

Richard Doyle