Anya Corazon (voice) / Chaperone (voice) / Female Clerk (voice)

Melanie Minichino

Max Modell (voice) / Trucker / Mr. Slott (voice)

Fred Tatasciore

Flash Thompson / Student #2 (voice) / Flash Thompson (voice)

Ben Diskin

Aunt May (voice) / Woman Bystander (voice) / Bomb Voice (voice)

Nancy Linari

Harry Osborn (voice) / Male Clerk / Guy in Line (voice)

Max Mittelman

Miles Morales (voice) / Security Robot (voice)

Nadji Jeter

Raymond Warren (voice) / Security Guard / Customer (voice)

John DiMaggio

Norman Osborn (voice) / Prison Guard (voice) / Hologram (voice)

Josh Keaton

Gwen Stacy (voice) / Osborn Recruiter #2 (voice)

Laura Bailey

Adrian Toomes (voice) / Thug #3 (voice)

Alastair Duncan

Peter Parker / Spider-Man (voice)

Robbie Daymond

Uncle Ben (voice)

Patton Oswalt

Doc Ock / Otto Octavius (voice)

Scott Menville

Hammerhead (voice)

Jim Cummings

Alistair Smythe (voice) / Audience Member (voice) / Alistair Smythe

Jason Spisak

Sand Girl (voice) / Keemia (voice)

Sofia Carson

Aleksei Sytsevich / Comic Book Guy (voice) / Rhino

Matthew Mercer

Herman Schultz / Shocker (voice)

Cameron Boyce

Prowler (voice)

Nathaniel J. Potvin

Police Officer #3 (voice)

Richard Doyle