Spider-Man
Robbie Daymond
Harry Osborn
Max Mittelman
Doc Ock
Scott Menville
Anya Corazon (voice) / Chaperone (voice) / Female Clerk (voice)
Melanie Minichino
Max Modell (voice) / Trucker / Mr. Slott (voice)
Fred Tatasciore
Miles Morales
Nadji Jeter
Flash Thompson / Student #2 (voice) / Flash Thompson (voice)
Ben Diskin
Aunt May (voice) / Woman Bystander (voice) / Bomb Voice (voice)
Nancy Linari
Gwen Stacy
Laura Bailey
Uncle Ben (voice)
Patton Oswalt
Raymond Warren (voice) / Security Guard / Customer (voice)
John DiMaggio
Norman Osborn (voice) / Prison Guard (voice) / Hologram (voice)
Josh Keaton
Vulture
Alastair Duncan
Bob Joles
Joe
Joe Quesada
Guest Voice
Nathaniel Potvin
April Stewart*
Guest Voice
Booboo Stewart
Hammerhead (voice)
Jim Cummings
Trevor Devall
Alistair Smythe (voice) / Audience Member (voice) / Alistair Smythe
Jason Spisak
Sand Girl (voice) / Keemia (voice)
Sofia Carson
Aleksei Sytsevich / Comic Book Guy (voice) / Rhino
Matthew Mercer
Herman Schultz / Shocker (voice)
Cameron Boyce
Police Officer #3 (voice)
Richard Doyle