Spider-Man

Robbie Daymond

Harry Osborn

Max Mittelman

Doc Ock

Scott Menville

Miles Morales (voice) / Security Robot (voice)

Nadji Jeter

Anya Corazon (voice) / Chaperone (voice) / Female Clerk (voice)

Melanie Minichino

Max Modell (voice) / Trucker / Mr. Slott (voice)

Fred Tatasciore

Ben Pronsky

Flash Thompson / Student #2 (voice) / Flash Thompson (voice)

Ben Diskin

Aunt May (voice) / Woman Bystander (voice) / Bomb Voice (voice)

Nancy Linari

Gwen Stacy

Laura Bailey

Uncle Ben

Patton Oswalt

Raymond Warren (voice) / Security Guard / Customer (voice)

John DiMaggio

Norman Osborn (voice) / Prison Guard (voice) / Hologram (voice)

Josh Keaton

Vulture

Alastair Duncan

Bob Joles

Joe

Joe Quesada

Guest Voice

Nathaniel Potvin

April Stewart

Guest Voice

Booboo Stewart

Trevor Devall

Hammerhead (voice)

Jim Cummings

Alistair Smythe (voice) / Audience Member (voice) / Alistair Smythe

Jason Spisak

Sand Girl (voice) / Keemia (voice)

Sofia Carson

Aleksei Sytsevich / Comic Book Guy (voice) / Rhino

Matthew Mercer

Herman Schultz / Shocker (voice)

Cameron Boyce

Police Officer #3 (voice)

Richard Doyle