Miles Morales

Shameik Moore

Gwen Stacey

Hailee Steinfeld

Miguel O'Hara (voice)

Oscar Isaac

Jeff Morales (voice)

Brian Tyree Henry

Peter B. Parker (voice)

Jake Johnson

Rio Morales

Luna Lauren Velez

Jessica Drew (voice)

Issa Rae

Jonathan Ohnn/The Spot

Jason Schwartzman

Ms. Weber (voice)

Rachel Dratch

Hobart "Hobie" Brown/Spider-Punk

Daniel Kaluuya

George Stacy (voice)

Shea Whigham

Ben Reilly/Scarlet Spider

Andy Samberg

Margo Kess: Spider-Byte (voice)

Amandla Stenberg

Pavitr Prabhakar (voice)

Karan Soni

LYLA (voice)

Greta Lee

Uncle Aaron (voice)

Mahershala Ali

Adrian Toomes/The Vulture

Jorma Taccone

Miles G. Morales (voice)

Jharrel Jerome

Peter Parker (voice)

Jack Quaid

Lenny (voice)

Ziggy Marley

J. Jonah Jameson (voice)

J.K. Simmons

Aaron Davis

Donald Glover

May / Quippy Spider-Person (voice)

Elizabeth Perkins

Doc Ock (voice)

Kathryn Hahn

Glory (voice)

Ayo Edibiri

MJ (voice)

Nicole Delaney

Betty (voice)

Antonina Lentini

Yuri (voice)

Atsuko Okatsuka

Ganke (voice)

Peter Sohn

Mary Jane (voice)

Melissa Sturm

Maria (voice)

Lorraine Velez

Lego Spider-Man (voice)

Nic Novicki

Web-Slinger (voice)

Taran Killam

Metro Spider-Man (voice)

Metro Boomin

Spectacular Spider-Man (voice)

Josh Keaton

Malala Windsor: Spider-UK (voice)

Sofia Barclay

Charlotte Webber: Sun-Spider (voice)

Danielle Perez

Insomniac Spider-Man (voice)

Yuri Lowenthal

Additional Voices (voice)

Rita Rani Ahuja

Additional Voices (voice)

Ismail Bashey

Additional Voices (voice)

Oscar Camacho

Additional Voices (voice)

Freddy Ferrari

Additional Voices (voice)

Kerry Gutierrez

Additional Voices (voice)

Kamal Khan

Additional Voices (voice)

Angelo Sekou Kouyate

Additional Voices (voice)

Andrew Leviton

Additional Voices (voice)

David Michie

Additional Voices (voice)

Sumit Naig

Additional Voices (voice)

Juan Pacheco

Additional Voices (voice)

Chrystee Pharris

Additional Voices (voice)

Ben Pronsky

Additional Voices (voice)

Al Rodrigo

Additional Voices (voice)

Stan Sellers

Additional Voices (voice)

Warren Sroka

Additional Voices (voice)

Jasper Johannes Andrews

Additional Voices (voice)

Gredel Berrios Calladine

Additional Voices (voice)

Natalia Castellanos

Additional Voices (voice)

Russell Tyre Francis

Additional Voices (voice)

Deepti Gupta

Additional Voices (voice)

Sohm Kapila

Additional Voices (voice)

Pradnya Kuwadekar

Additional Voices (voice)

Ashley London

Additional Voices (voice)

Christopher Miller

Additional Voices (voice)

Andrea Navedo

Additional Voices (voice)

Lakshmi Patel

Additional Voices (voice)

Jacqueline Pinol

Additional Voices (voice)

Eliyas Qureshi

Additional Voices (voice)

Lashana Rodriguez

Additional Voices (voice)

Jaswant Dev Shrestha

Additional Voices (voice)

Libby Thomas Dickey

Additional Voices (voice)

Jason Linere-White

Additional Voices (voice)

Sitara Attaie

Additional Voices (voice)

Mayuri Bhandari

Additional Voices (voice)

June Christopher

Additional Voices (voice)

Michelle Jubilee Gonzalez

Additional Voices (voice)

Marabina Jaimes

Additional Voices (voice)

Rez Kempton

Additional Voices (voice)

Lex Lang

Additional Voices (voice)

Phil Lord

Additional Voices (voice)

Richard Miro

Additional Voices (voice)

Doug Nicholas

Additional Voices (voice)

Shakira Ja'nai Paye

Additional Voices (voice)

James Pirri

Additional Voices (voice)

Marley Ralph

Additional Voices (voice)

Michelle Ruff

Additional Voices (voice)

Dennis Singletary

Additional Voices (voice)

Amanda Troop

Additional Voices (voice)

Ruth Zalduondo

Additional Voices (voice)

Kimberly Bailey

Additional Voices (voice)

Sanjay Chandani

Additional Voices (voice)

Melanie Duke

Additional Voices (voice)

Jorge R. Gutierrez

Additional Voices (voice)

Miguel Jiron

Additional Voices (voice)

Deepti Kingra-Mickelsen

Additional Voices (voice)

Luisa Leschin

Additional Voices (voice)

Caitlin McKenna-Wilkinson

Additional Voices (voice)

Andrew Morgado

Additional Voices (voice)

Arthur Ortiz

Additional Voices (voice)

Eliana A. Perez

Additional Voices (voice)

Juan Pope

Additional Voices (voice)

Michael Rianda

Additional Voices (voice)

Erika Scopelli

Additional Voices (voice)

Narender Sood

Additional Voices (voice)

Cedric L. Williams